In view of the continuing global concerns with antibiotic resistance, there is a pressing need to have a scientific forum to assess the scientific advancements made since the 1st International Symposium on Alternatives to Antibiotics held in 2012. The objectives of this 2nd International Symposium are to highlight promising research results and novel technologies that provide alternatives to antibiotics for use in animal health and production, assess challenges associated with their commercialization and use, and provide actionable strategies to support their development. The symposium will focus on five product categories that could reduce the use of medically important antibiotics in animal health and production: 1) vaccines; 2) microbial-derived products; 3) phytochemicals; 4) immune-derived products; and 5) chemicals.